Hotspot

ycf1

SSC · 125226-130883 · 5658 bp

Back to Hippophae

Pi 0.0107

MarkerSeek score 52.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5658
variable_sites: 269
pi: 0.0107
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGATAGGAATACCCTCTAGCA	AAGGCGGATTCATTATGGAA	42.9	40.0	54.7	54.8	223 bp	1.0	48.0
ycf1_p2	TTGATAGGAATACCCTCTAGCA	AAGGCGGATTCATTATGGAA	40.9	40.0	55.5	54.8	224 bp	1.0	48.0
ycf1_p3	TGATAGGAATACCCTCTAGCA	AGGCGGATTCATTATGGAAT	42.9	40.0	54.7	54.5	222 bp	1.0	48.0
ycf1_p4	TGATAGGAATACCCTCTAGCA	AAGGCGGATTCATTATGGAAT	42.9	38.1	54.7	55.4	223 bp	1.0	48.0
ycf1_p5	TGATAGGAATACCCTCTAGCAT	AAGGCGGATTCATTATGGAA	40.9	40.0	55.3	54.8	223 bp	1.0	48.0