Hotspot

trnR-ACG-rrn5

IRa · 131939-132190 · 252 bp

Back to Hippophae

Pi 0.0522
MarkerSeek score 81.70
Primer available yes
Label trnR-ACG-rrn5

Feature Metadata

label_name
trnR-ACG-rrn5
feature_type
igs
region
IRa
length_bp
252
variable_sites
34
pi
0.0522
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-rrn5_p1 CCAAAGGGAAAGGTCCTTCC GGTGACGATACTGTAGGGGA 55.0 55.0 58.1 57.9 408-420 bp 1.0 86.6
trnR-ACG-rrn5_p2 CCAAAGGGAAAGGTCCTTCC AATCCATCCCGAACTTGGTG 55.0 50.0 58.1 57.9 443-455 bp 1.0 85.5
trnR-ACG-rrn5_p3 CCAAAGGGAAAGGTCCTTCC ACCAATCCATCCCGAACTTG 55.0 50.0 58.1 57.9 446-458 bp 1.0 85.5
trnR-ACG-rrn5_p4 CCAAAGGGAAAGGTCCTTCC CAATCCATCCCGAACTTGGT 55.0 50.0 58.1 57.9 444-456 bp 1.0 85.5
trnR-ACG-rrn5_p5 CCAAAGGGAAAGGTCCTTCC CACCAATCCATCCCGAACTT 55.0 50.0 58.1 57.9 447-459 bp 1.0 85.4