Hotspot

rpl32-trnL-UAG

SSC · 114345-115526 · 1182 bp

Back to Hippophae

Pi 0.029

MarkerSeek score 74.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1182
variable_sites: 133
pi: 0.029
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGCATATTGGGCAGCATTGA	ATAACACTTGGCGCGGTTAT	45.0	45.0	57.9	58.0	1583-1792 bp	1.0	90.9
rpl32-trnL-UAG_p2	AGCATATTGGGCAGCATTGA	AAAAATAACACTTGGCGCGG	45.0	45.0	57.9	57.9	1587-1796 bp	1.0	90.6
rpl32-trnL-UAG_p3	TAAGGATTAGGGGTTGGGGG	ATAACACTTGGCGCGGTTAT	55.0	45.0	58.1	58.0	2134-2342 bp	0.461538	63.3
rpl32-trnL-UAG_p4	AGCATATTGGGCAGCATTGA	ACTTGGCGCGGTTATTACAT	45.0	45.0	57.9	58.0	1778 bp	0.076923	42.2
rpl32-trnL-UAG_p5	TAAGGATTAGGGGTTGGGGG	ACTTGGCGCGGTTATTACAT	55.0	45.0	58.1	58.0	2322 bp	0.076923	42.1