Hotspot

ndhF-rpl32

SSC · 112919-114188 · 1270 bp

Back to Hippophae

Pi 0.0265
MarkerSeek score 75.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1270
variable_sites
143
pi
0.0265
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CCCTACGCGATTGGTTACAA TCAATGCTGCCCAATATGCT 50.0 45.0 58.0 57.9 1652-1939 bp 1.0 90.4
ndhF-rpl32_p2 AACAGGTCGTGTGAACCAAA TCAATGCTGCCCAATATGCT 45.0 45.0 57.9 57.9 1607-1894 bp 1.0 89.7
ndhF-rpl32_p3 ACAGGTCGTGTGAACCAAAA TCAATGCTGCCCAATATGCT 45.0 45.0 57.9 57.9 1606-1893 bp 1.0 89.7
ndhF-rpl32_p4 ATTGGGCGGATTTAGCAACT TCAATGCTGCCCAATATGCT 45.0 45.0 57.9 57.9 1827-2114 bp 1.0 89.1
ndhF-rpl32_p5 CCCTACGCGATTGGTTACAA AAAAGGCTTTCAATGCTGCC 50.0 45.0 58.0 57.8 1661-1948 bp 0.923077 86.3