Hotspot

rrn5-trnR-ACG

IRb · 108157-108408 · 252 bp

Back to Hippophae

Pi 0.0526

MarkerSeek score 81.70

Primer available yes

Label rrn5-trnR-ACG

Feature Metadata

label_name: rrn5-trnR-ACG
feature_type: igs
region: IRb
length_bp: 252
variable_sites: 34
pi: 0.0526
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-trnR-ACG_p1	GGTGACGATACTGTAGGGGA	CCAAAGGGAAAGGTCCTTCC	55.0	55.0	57.9	58.1	408-420 bp	1.0	86.6
rrn5-trnR-ACG_p2	AATCCATCCCGAACTTGGTG	CCAAAGGGAAAGGTCCTTCC	50.0	55.0	57.9	58.1	443-455 bp	1.0	85.5
rrn5-trnR-ACG_p3	ACCAATCCATCCCGAACTTG	CCAAAGGGAAAGGTCCTTCC	50.0	55.0	57.9	58.1	446-458 bp	1.0	85.5
rrn5-trnR-ACG_p4	CAATCCATCCCGAACTTGGT	CCAAAGGGAAAGGTCCTTCC	50.0	55.0	57.9	58.1	444-456 bp	1.0	85.5
rrn5-trnR-ACG_p5	CACCAATCCATCCCGAACTT	CCAAAGGGAAAGGTCCTTCC	50.0	55.0	57.9	58.1	447-459 bp	1.0	85.4