Hotspot

rps8-rpl14

LSC · 80272-80449 · 178 bp

Back to Hippophae

Pi 0.029

MarkerSeek score 72.00

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 178
variable_sites: 16
pi: 0.029
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	AGATGGTACTCGAACTGTTCT	CGCCCGGGAATTAAGACAAT	42.9	50.0	56.1	58.3	321-327 bp	1.0	60.6
rps8-rpl14_p2	TGTCAGCATTTCGTATAGAGGT	CGCCCGGGAATTAAGACAAT	40.9	50.0	57.0	58.3	289-295 bp	1.0	60.2
rps8-rpl14_p3	AGATGGTACTCGAACTGTTCT	GGGAACTCGAATTTTTGGCG	42.9	50.0	56.1	58.0	345-351 bp	0.923077	59.2
rps8-rpl14_p4	TGTCAGCATTTCGTATAGAGGT	GGGAACTCGAATTTTTGGCG	40.9	50.0	57.0	58.0	313-319 bp	0.923077	58.8
rps8-rpl14_p5	AGATGGTACTCGAACTGTTCTT	GGGAACTCGAATTTTTGGCG	40.9	50.0	56.9	58.0	345-351 bp	0.923077	57.1