Hotspot

rbcL-accD

LSC · 56667-57594 · 928 bp

Back to Hippophae

Pi 0.0155

MarkerSeek score 75.60

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 928
variable_sites: 61
pi: 0.0155
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGTCCTGAACTAGCTGCTG	AGGACCACCAAGACTATCCA	55.0	50.0	57.7	57.4	947-1073 bp	1.0	81.6
rbcL-accD_p2	TTGCTCGTGAGGGTAATGAC	AGGACCACCAAGACTATCCA	50.0	50.0	57.6	57.4	990-1116 bp	1.0	80.6
rbcL-accD_p3	GCTGCTGCTTGTGAAGTATG	AGGACCACCAAGACTATCCA	50.0	50.0	57.5	57.4	934-1060 bp	1.0	79.6
rbcL-accD_p4	GCTCGTGAGGGTAATGACAT	AGGACCACCAAGACTATCCA	50.0	50.0	57.4	57.4	988-1114 bp	1.0	78.9
rbcL-accD_p5	CTTGCTCGTGAGGGTAATGA	AGGACCACCAAGACTATCCA	50.0	50.0	57.3	57.4	991-1117 bp	1.0	78.3