Pi
0.0179
MarkerSeek score
71.60
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 664-846 bp | 1.0 | 88.9 |
| trnT-UGU-trnL-UAA_p2 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 672-854 bp | 1.0 | 88.3 |
| trnT-UGU-trnL-UAA_p3 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 696-878 bp | 1.0 | 87.8 |
| trnT-UGU-trnL-UAA_p4 | ATGGAGAAAAGCCGGCTATC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.7 | 58.0 | 716-898 bp | 1.0 | 87.5 |
| trnT-UGU-trnL-UAA_p5 | GATGGAGAAAAGCCGGCTAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.7 | 58.0 | 717-899 bp | 1.0 | 87.5 |