Hotspot

psbZ-trnG-UCC

LSC · 35577-36118 · 542 bp

Back to Hippophae

Pi 0.0275

MarkerSeek score 79.40

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 542
variable_sites: 63
pi: 0.0275
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	TTGCTTCTCCTGATGGTTGG	AATCGAACCCGCATCTTCTC	50.0	50.0	57.8	58.1	704-721 bp	1.0	88.1
psbZ-trnG-UCC_p2	TTGCTTCTCCTGATGGTTGG	GAATCGAACCCGCATCTTCT	50.0	50.0	57.8	58.1	705-722 bp	1.0	88.1
psbZ-trnG-UCC_p3	TGGCCGGGTAACAAAAATGT	AATCGAACCCGCATCTTCTC	45.0	50.0	58.3	58.1	687-704 bp	1.0	87.7
psbZ-trnG-UCC_p4	TGGCCGGGTAACAAAAATGT	GAATCGAACCCGCATCTTCT	45.0	50.0	58.3	58.1	688-705 bp	1.0	87.7
psbZ-trnG-UCC_p5	TTGGACTGGTCTTTCTGGTG	AATCGAACCCGCATCTTCTC	50.0	50.0	57.4	58.1	641-658 bp	1.0	85.2