Hotspot

trnC-GCA-petN

LSC · 27999-28829 · 831 bp

Back to Hippophae

Pi 0.0228

MarkerSeek score 72.10

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 831
variable_sites: 86
pi: 0.0228
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	781-983 bp	1.0	90.0
trnC-GCA-petN_p2	GGGGACTGCAAATCCTCTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.8	58.2	799-1001 bp	1.0	88.7
trnC-GCA-petN_p3	TTTTGTATCGTTTTGGCGGC	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.9	58.2	838-958 bp	0.923077	85.7
trnC-GCA-petN_p4	TGCAAATCCTCTTTTCCCCA	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.3	58.2	793-995 bp	1.0	85.0
trnC-GCA-petN_p5	GGTGTCGCCTGATCAACAAA	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.8	58.2	762-964 bp	0.923077	80.7