Hotspot

rpoB-trnC-GCA

LSC · 26640-27913 · 1274 bp

Back to Hippophae

Pi 0.0326

MarkerSeek score 78.10

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1274
variable_sites: 130
pi: 0.0326
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCATTCCCACCTCCAAGCAT	CCGGATTTGAACTGGGGAAA	50.0	50.0	59.0	58.1	1263-1359 bp	1.0	83.2
rpoB-trnC-GCA_p2	TCATTCCCACCTCCAAGCAT	AAAGAGGATTTGCAGTCCCC	50.0	50.0	59.0	57.8	1245-1341 bp	1.0	82.1
rpoB-trnC-GCA_p3	TTCATTCCCACCTCCAAGCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	59.2	58.1	1264-1360 bp	1.0	81.3
rpoB-trnC-GCA_p4	TTCATTCCCACCTCCAAGCA	AAAGAGGATTTGCAGTCCCC	50.0	50.0	59.2	57.8	1246-1342 bp	1.0	80.3
rpoB-trnC-GCA_p5	TCATTCCCACCTCCAAGCA	CCGGATTTGAACTGGGGAAA	52.6	50.0	58.5	58.1	1263-1359 bp	1.0	78.9