Hotspot

trnR-UCU-atpA

LSC · 9721-9969 · 249 bp

Back to Hippophae

Pi 0.0227

MarkerSeek score 66.80

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 249
variable_sites: 23
pi: 0.0227
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGAATGAAAAGCGTCCATTGT	AGCACTTTTGAAAGAGGCGA	42.9	45.0	57.4	58.0	383-401 bp	1.0	76.0
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	AGCACTTTTGAAAGAGGCGA	47.6	45.0	57.3	58.0	323-341 bp	1.0	75.7
trnR-UCU-atpA_p3	GGAATGAAAAGCGTCCATTGT	CATTTACGGGGGAAGCAGAA	42.9	50.0	57.4	57.9	402-420 bp	1.0	75.1
trnR-UCU-atpA_p4	GGAATGAAAAGCGTCCATTGT	AAGACATTTACGGGGGAAGC	42.9	50.0	57.4	57.9	406-424 bp	1.0	75.1
trnR-UCU-atpA_p5	GGTTCAAATCCTATTGGACGC	CATTTACGGGGGAAGCAGAA	47.6	50.0	57.3	57.9	342-360 bp	1.0	74.9