Hotspot

ycf1

SSC · 120050-125428 · 5379 bp

Back to Hippocrepis

Pi 0.027
MarkerSeek score 55.10
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5379
variable_sites
217
pi
0.027
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TCTTATGAATTAATGGGAGGCG AGACATTATGATGATTCAAAATGAGA 40.9 26.9 56.0 55.3 170 bp 1.0 46.1
ycf1_p2 TCTTATGAATTAATGGGAGGCG AGTCTAGACATTATGATGATTCA 40.9 30.4 56.0 52.5 175 bp 1.0 45.9
ycf1_p3 TCTTATGAATTAATGGGAGGCG AGTCTAGACATTATGATGATTCAA 40.9 29.2 56.0 53.3 175 bp 1.0 45.9
ycf1_p4 TTCTTATGAATTAATGGGAGGCG AGTCTAGACATTATGATGATTCA 39.1 30.4 56.7 52.5 176 bp 1.0 45.9
ycf1_p5 TCTTATGAATTAATGGGAGGCG AGTCTAGACATTATGATGATTCAAA 40.9 28.0 56.0 54.1 175 bp 1.0 45.9