Hotspot

pafI

LSC · 16217-18126 · 1910 bp

Back to Hippocrepis

Pi 0.0108

MarkerSeek score 53.20

Primer available yes

Label pafI

Feature Metadata

label_name: pafI
feature_type: gene
region: LSC
length_bp: 1910
variable_sites: 31
pi: 0.0108
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafI_p1	CCACTCAGCCATCTCTCCTA	ACACATACAAAGAGACCCGC	55.0	50.0	57.9	57.9	2692-2996 bp	1.0	83.2
pafI_p2	CCACTCAGCCATCTCTCCTA	CATACAAAGAGACCCGCCAA	55.0	50.0	57.9	57.9	2689-2993 bp	1.0	83.1
pafI_p3	CCACTCAGCCATCTCTCCTA	GCATCAGCATGTAGGTTCCA	55.0	50.0	57.9	58.0	2872-2903 bp	0.666667	67.3
pafI_p4	CCACTCAGCCATCTCTCCTA	TCAGGCCCCTTAGCTATTGT	55.0	50.0	57.9	58.1	2836-2867 bp	0.666667	66.8
pafI_p5	CCACTCAGCCATCTCTCCTA	TGCCCATTCCTCGAAAGAAG	55.0	50.0	57.9	57.9	2795-2826 bp	0.666667	66.7