Hotspot

ndhF-rpl32

SSC · 108910-109629 · 720 bp

Back to Hippocrepis

Pi 0.0337
MarkerSeek score 74.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
720
variable_sites
35
pi
0.0337
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ATATAGGAACGGGGAGTGGG TGCTGTCCAATATCCCCTCT 55.0 50.0 58.0 57.8 865-896 bp 1.0 82.7
ndhF-rpl32_p2 GGGGAGTGGGATGAAAGGTA TGCTGTCCAATATCCCCTCT 55.0 50.0 58.4 57.8 855-886 bp 1.0 79.7
ndhF-rpl32_p3 ATATAGGAACGGGGAGTGGG TCAATGCTGTCCAATATCCCC 55.0 47.6 58.0 57.8 869-900 bp 1.0 74.4
ndhF-rpl32_p4 ATATAGGAACGGGGAGTGGG CAATGCTGTCCAATATCCCCT 55.0 47.6 58.0 57.8 868-899 bp 1.0 74.4
ndhF-rpl32_p5 TATAGGAACGGGGAGTGGGA TGCTGTCCAATATCCCCTCT 55.0 50.0 59.1 57.8 864-895 bp 1.0 74.4