Hotspot

rps11-rpl36

LSC · 76519-76860 · 342 bp

Back to Hippocrepis

Pi 0.0445

MarkerSeek score 73.50

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 342
variable_sites: 22
pi: 0.0445
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CGTGCATTTTTACGTGAACCA	AGGCGAGGTCGGATTATAGT	42.9	50.0	58.0	57.7	446-493 bp	1.0	75.1
rps11-rpl36_p2	CGTGCATTTTTACGTGAACCA	CAGGCGAGGTCGGATTATAG	42.9	55.0	58.0	57.4	447-494 bp	1.0	72.3
rps11-rpl36_p3	CGTGCATTTTTACGTGAACC	AGGCGAGGTCGGATTATAGT	45.0	50.0	56.3	57.7	446-493 bp	1.0	69.4
rps11-rpl36_p4	CGTGCATTTTTACGTGAACCA	AAATAGGAGCGTCTGTTCGT	42.9	45.0	58.0	57.0	496-543 bp	1.0	68.7
rps11-rpl36_p5	CGTGCATTTTTACGTGAACCA	TAGGAGCGTCTGTTCGTAAG	42.9	50.0	58.0	56.8	493-540 bp	1.0	67.4