Hotspot

trnP-UGG-psaJ

LSC · 63249-63931 · 683 bp

Back to Hippocrepis

Pi 0.0377

MarkerSeek score 75.10

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 683
variable_sites: 37
pi: 0.0377
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	GTGCCACGGAGAGATATGTT	50.0	50.0	58.1	57.4	762-775 bp	1.0	81.3
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	GTGCCACGGAGAGATATGTT	50.0	50.0	58.1	57.4	761-774 bp	1.0	81.3
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	GTGCCACGGAGAGATATGTT	50.0	50.0	58.1	57.4	773-786 bp	1.0	81.2
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	GTGCCACGGAGAGATATGTT	50.0	50.0	58.1	57.4	772-785 bp	1.0	81.2
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	GTGCCACGGAGAGATATGTT	50.0	50.0	58.1	57.4	775-788 bp	1.0	81.1