Hotspot

psbK-trnQ-UUG

LSC · 53123-53687 · 565 bp

Back to Hippocrepis

Pi 0.0597

MarkerSeek score 85.70

Primer available yes

Label psbK-trnQ-UUG

Feature Metadata

label_name: psbK-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 565
variable_sites: 48
pi: 0.0597
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 85.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-trnQ-UUG_p1	ACTTGAATAAGGGGCAGAGT	GACCAAAACCCGTTGCCTTA	45.0	50.0	56.1	58.7	656-661 bp	1.0	69.3
psbK-trnQ-UUG_p2	ACTTGAATAAGGGGCAGAGTT	GACCAAAACCCGTTGCCTTA	42.9	50.0	56.9	58.7	656-661 bp	1.0	67.6
psbK-trnQ-UUG_p3	ACTTGAATAAGGGGCAGAGT	GAATAGCGGGACCAAAACCC	45.0	55.0	56.1	58.9	665-670 bp	1.0	67.5
psbK-trnQ-UUG_p4	ACTTGAATAAGGGGCAGAGT	ACCAAAACCCGTTGCCTTAC	45.0	50.0	56.1	59.0	655-660 bp	1.0	67.1
psbK-trnQ-UUG_p5	ACTTGAATAAGGGGCAGAGT	ATATGCTCTGGGACGGAAGG	45.0	55.0	56.1	58.9	697-702 bp	1.0	66.9