Hotspot

trnG-UCC-trnS-GCU

LSC · 51437-52152 · 716 bp

Back to Hippocrepis

Pi 0.0335
MarkerSeek score 69.00
Primer available yes
Label trnG-UCC-trnS-GCU

Feature Metadata

label_name
trnG-UCC-trnS-GCU
feature_type
igs
region
LSC
length_bp
716
variable_sites
36
pi
0.0335
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnS-GCU_p1 TCAGGGAGTCCATAGAACGA CAATCCGACGCTTTAGTCCA 50.0 50.0 57.2 58.0 801-917 bp 1.0 76.9
trnG-UCC-trnS-GCU_p2 TCAGGGAGTCCATAGAACGA AATCCGACGCTTTAGTCCAC 50.0 50.0 57.2 58.0 800-916 bp 1.0 76.9
trnG-UCC-trnS-GCU_p3 GGGAGTCCATAGAACGAATCG CAATCCGACGCTTTAGTCCA 52.4 50.0 58.0 58.0 798-914 bp 1.0 75.4
trnG-UCC-trnS-GCU_p4 GGGAGTCCATAGAACGAATCG AATCCGACGCTTTAGTCCAC 52.4 50.0 58.0 58.0 797-913 bp 1.0 75.4
trnG-UCC-trnS-GCU_p5 GAGTCCATAGAACGAATCGCA CAATCCGACGCTTTAGTCCA 47.6 50.0 57.9 58.0 796-912 bp 1.0 74.9