Hotspot

atpA-trnR-UCU

LSC · 50251-50366 · 116 bp

Back to Hippocrepis

Pi 0.058

MarkerSeek score 82.00

Primer available yes

Label atpA-trnR-UCU

Feature Metadata

label_name: atpA-trnR-UCU
feature_type: igs
region: LSC
length_bp: 116
variable_sites: 9
pi: 0.058
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-trnR-UCU_p1	CATTCACTGGGGAAGCAGAA	TTCAAATCCTATTGGGCGCA	50.0	45.0	57.8	57.9	223-404 bp	1.0	82.9
atpA-trnR-UCU_p2	AAGACATTCACTGGGGAAGC	TTCAAATCCTATTGGGCGCA	50.0	45.0	57.8	57.9	227-408 bp	1.0	82.8
atpA-trnR-UCU_p3	ATTCACTGGGGAAGCAGAAG	TTCAAATCCTATTGGGCGCA	50.0	45.0	57.5	57.9	222-403 bp	1.0	80.5
atpA-trnR-UCU_p4	GACATTCACTGGGGAAGCAG	TTCAAATCCTATTGGGCGCA	55.0	45.0	58.5	57.9	225-406 bp	1.0	80.1
atpA-trnR-UCU_p5	ACCAAGACATTCACTGGGGA	TTCAAATCCTATTGGGCGCA	50.0	45.0	58.6	57.9	230-411 bp	1.0	79.9