Hotspot

pafI-psaA

LSC · 18127-18670 · 544 bp

Back to Hippocrepis

Pi 0.0631

MarkerSeek score 84.30

Primer available yes

Label pafI-psaA

Feature Metadata

label_name: pafI-psaA
feature_type: igs
region: LSC
length_bp: 544
variable_sites: 43
pi: 0.0631
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafI-psaA_p1	TGGTTAAAGATTACAAGGCGT	TGCCCATTCCTCGAAAGAAG	38.1	50.0	55.5	57.9	619-947 bp	1.0	59.9
pafI-psaA_p2	TGGTTAAAGATTACAAGGCGT	GCCCATTCCTCGAAAGAAGT	38.1	50.0	55.5	57.9	618-946 bp	1.0	59.8
pafI-psaA_p3	TGGTTAAAGATTACAAGGCGT	TTGAGAAATGGCCGGGTTTT	38.1	45.0	55.5	58.3	638-966 bp	1.0	58.3
pafI-psaA_p4	TGGTTAAAGATTACAAGGCGTT	TGCCCATTCCTCGAAAGAAG	36.4	50.0	56.3	57.9	619-947 bp	1.0	57.9
pafI-psaA_p5	TTGGTTAAAGATTACAAGGCGT	TGCCCATTCCTCGAAAGAAG	36.4	50.0	56.3	57.9	620-948 bp	1.0	57.9