Hotspot

trnH-GUG-psbA

LSC · 124-404 · 281 bp

Back to Hippocrepis

Pi 0.11

MarkerSeek score 77.40

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 281
variable_sites: 37
pi: 0.11
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CGGTCGAGGCTCCATCTATA	55.0	55.0	58.1	58.2	275-352 bp	1.0	89.5
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CGGTCGAGGCTCCATCTATA	50.0	55.0	57.9	58.2	289-366 bp	1.0	89.5
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CGGTCGAGGCTCCATCTATA	50.0	55.0	57.9	58.2	288-365 bp	1.0	89.5
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CGGTCGAGGCTCCATCTATA	50.0	55.0	57.9	58.2	290-367 bp	1.0	89.5
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	ACTTCCCTCTAGACCTAGCG	55.0	55.0	58.1	57.6	296-373 bp	1.0	88.2