Hotspot

psbA

LSC · 86-1147 · 1062 bp

Back to Hippeastrum

Pi 0.000732

MarkerSeek score 29.90

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 2
pi: 0.000732
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TGGGTATGGCTCCTTAAACG	TTGCGAAGATTAGGTTCGGG	50.0	50.0	57.3	58.0	1812-1814 bp	0.888889	70.2
psbA_p2	TGGGTATGGCTCCTTAAACG	GTGCTTGGGAGTCCTTGAAA	50.0	50.0	57.3	58.0	1151 bp	0.888889	69.9
psbA_p3	TGGGTATGGCTCCTTAAACG	CATAGGGAAAGTCGTGTGCA	50.0	50.0	57.3	57.9	1475 bp	0.888889	69.6
psbA_p4	TGGGTATGGCTCCTTAAACG	ACATAGGGAAAGTCGTGTGC	50.0	50.0	57.3	57.9	1476 bp	0.888889	69.6
psbA_p5	TGGGTATGGCTCCTTAAACG	GTTTGGGGAGGGGTCTTTTT	50.0	50.0	57.3	57.9	1437 bp	0.888889	69.5