Hotspot

psbB-psbT

LSC · 76261-76450 · 190 bp

Back to Hippeastrum

Pi 0.0076

MarkerSeek score 51.00

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 190
variable_sites: 5
pi: 0.0076
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	GCGCTAGAACCTTGTTCAGA	CCTTAGGCGGTTCTCGAAAA	50.0	50.0	57.9	57.9	390-394 bp	1.0	80.5
psbB-psbT_p2	GCGCTAGAACCTTGTTCAGA	TTTAGTCGGAACCTTAGGCG	50.0	50.0	57.9	57.3	401-405 bp	1.0	75.9
psbB-psbT_p3	GGCGCTAGAACCTTGTTCAG	CCTTAGGCGGTTCTCGAAAA	55.0	50.0	58.9	57.9	391-395 bp	1.0	73.8
psbB-psbT_p4	GCGCTAGAACCTTGTTCAGA	AACCTTAGGCGGTTCTCGAA	50.0	50.0	57.9	59.0	392-396 bp	1.0	73.0
psbB-psbT_p5	GCGCTAGAACCTTGTTCAGA	ACCTTAGGCGGTTCTCGAAA	50.0	50.0	57.9	59.0	391-395 bp	1.0	73.0