Hotspot

trnT-UGU-trnL-UAA

LSC · 47761-48770 · 1010 bp

Back to Hippeastrum

Pi 0.00132
MarkerSeek score 57.70
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
1010
variable_sites
6
pi
0.00132
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 55.0 50.0 57.9 58.0 1035-1090 bp 1.0 83.4
trnT-UGU-trnL-UAA_p2 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 55.0 50.0 57.9 58.1 1043-1098 bp 1.0 82.8
trnT-UGU-trnL-UAA_p3 GGAATCGAACCGATGACCAT ACCAATTTCGCCATATCCCC 50.0 50.0 57.8 58.0 1067-1122 bp 1.0 82.5
trnT-UGU-trnL-UAA_p4 GCGATGCTCTAACCTCTGAG GGAAGTTCCCACTTAGCAGG 55.0 55.0 57.9 57.9 1096-1151 bp 1.0 82.4
trnT-UGU-trnL-UAA_p5 GGAATCGAACCGATGACCAT TAGCGTCTACCAATTTCGCC 50.0 50.0 57.8 58.1 1075-1130 bp 1.0 82.0