Hotspot

psbM-trnD-GUC

LSC · 30711-31750 · 1040 bp

Back to Hippeastrum

Pi 0.00123

MarkerSeek score 47.60

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1040
variable_sites: 5
pi: 0.00123
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	AGTTCAATCGGTCAGAGCAC	45.0	50.0	55.8	57.9	1151-1154 bp	1.0	62.0
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	TACACCTCACCGGGATTGTA	45.0	50.0	55.8	57.8	1170-1173 bp	1.0	60.8
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	CCTCACCGGGATTGTAGTTC	45.0	55.0	55.8	57.7	1166-1169 bp	1.0	60.2
psbM-trnD-GUC_p4	AGAATGAACAGTGCAGTAGC	ACCTCACCGGGATTGTAGTT	45.0	50.0	55.8	58.3	1167-1170 bp	1.0	59.9
psbM-trnD-GUC_p5	AGAATGAACAGTGCAGTAGC	ATACACCTCACCGGGATTGT	45.0	50.0	55.8	58.4	1171-1174 bp	1.0	59.3