Hotspot

atpF-atpH

LSC · 13898-14392 · 495 bp

Back to Hippeastrum

Pi 0.00595

MarkerSeek score 41.80

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 495
variable_sites: 8
pi: 0.00595
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	GTCGTGGCATTAGCACTTTT	50.0	45.0	57.9	57.0	604-607 bp	1.0	72.6
atpF-atpH_p2	CAGTGGCCTAAGGAAACGAA	GTCGTGGCATTAGCACTTTT	50.0	45.0	57.8	57.0	576-579 bp	1.0	72.2
atpF-atpH_p3	AGTGGCCTAAGGAAACGAAA	GTCGTGGCATTAGCACTTTT	45.0	45.0	57.1	57.0	575-578 bp	1.0	66.1
atpF-atpH_p4	CCAGTGGCCTAAGGAAACG	GTCGTGGCATTAGCACTTTT	57.9	45.0	58.1	57.0	577-580 bp	1.0	64.4
atpF-atpH_p5	AGTGGCCTAAGGAAACGAAAG	GTCGTGGCATTAGCACTTTT	47.6	45.0	58.2	57.0	575-578 bp	1.0	63.8