Hotspot

ycf3-trnS-GGA

LSC · 46305-46897 · 593 bp

Back to Himalayacalamus

Pi 0.0622
MarkerSeek score 91.00
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
593
variable_sites
36
pi
0.0622
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
91.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TTTTTCCCCTGAGGTTGTCG TTCCAATGCTACGCCTTGAA 50.0 45.0 58.0 57.8 728-730 bp 1.0 83.8
ycf3-trnS-GGA_p2 TTTTTCCCCTGAGGTTGTCG ACGGAAAGAGAGGGATTCGA 50.0 50.0 58.0 58.2 778-780 bp 1.0 83.7
ycf3-trnS-GGA_p3 TTTTTCCCCTGAGGTTGTCG AGTTCCAATGCTACGCCTTG 50.0 50.0 58.0 58.5 730-732 bp 1.0 80.9
ycf3-trnS-GGA_p4 TTTTTCCCCTGAGGTTGTCG CAGTTCCAATGCTACGCCTT 50.0 50.0 58.0 58.5 731-733 bp 1.0 80.9
ycf3-trnS-GGA_p5 TTTTTCCCCTGAGGTTGTCG TCCAATGCTACGCCTTGAAC 50.0 50.0 58.0 58.6 727-729 bp 1.0 80.9