Hotspot

psaA-ycf3

LSC · 43689-44312 · 624 bp

Back to Himalayacalamus

Pi 0.00321

MarkerSeek score 60.80

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 624
variable_sites: 2
pi: 0.00321
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGTTCTTGAGAAATGGCCGG	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.5	58.0	804-805 bp	1.0	76.5
psaA-ycf3_p2	TTTTACTTCTGGTTCCGGCG	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.5	58.0	732-733 bp	1.0	76.3
psaA-ycf3_p3	TGTTCTTGAGAAATGGCCGG	GCGCTTACTCCGGGAAATTA	50.0	50.0	58.5	58.1	800-801 bp	1.0	76.1
psaA-ycf3_p4	TTTTACTTCTGGTTCCGGCG	GCGCTTACTCCGGGAAATTA	50.0	50.0	58.5	58.1	728-729 bp	1.0	76.0
psaA-ycf3_p5	GGTTTGGCCCATTCCTCAAA	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.7	58.0	785-786 bp	1.0	75.0