Hotspot

trnS-GCU-psbD

LSC · 8470-9450 · 981 bp

Back to Himalayacalamus

Pi 0.00306
MarkerSeek score 48.80
Primer available yes
Label trnS-GCU-psbD

Feature Metadata

label_name
trnS-GCU-psbD
feature_type
igs
region
LSC
length_bp
981
variable_sites
3
pi
0.00306
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-psbD_p1 ATTAGCAATCCGCCGCTTTA ACTCTACCAAGGGCTATAGTCA 45.0 45.5 58.0 57.5 1044 bp 1.0 59.7
trnS-GCU-psbD_p2 GCTTTAGTCCACTCAGCCAT ACTCTACCAAGGGCTATAGTCA 50.0 45.5 57.6 57.5 1030 bp 1.0 56.7
trnS-GCU-psbD_p3 AGTCCACTCAGCCATCTCTC ACTCTACCAAGGGCTATAGTCA 55.0 45.5 58.5 57.5 1025 bp 1.0 55.9
trnS-GCU-psbD_p4 ATTAGCAATCCGCCGCTTTA ACTCTACCAAGGGCTATAGTCAT 45.0 43.5 58.0 57.9 1044 bp 1.0 55.4
trnS-GCU-psbD_p5 ATTAGCAATCCGCCGCTTTA AACTCTACCAAGGGCTATAGTCA 45.0 43.5 58.0 58.1 1045 bp 1.0 55.0