Hotspot

trnK-UUU-rps16

LSC · 3966-4521 · 556 bp

Back to Himalayacalamus

Pi 0.0054
MarkerSeek score 60.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
556
variable_sites
3
pi
0.0054
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCGCACTTAAAAGCCGAG GGTGCTCAACCTACAAGAACT 50.0 47.6 58.6 57.9 690 bp 1.0 66.8
trnK-UUU-rps16_p2 TAGTCGCACTTAAAAGCCGA GGTGCTCAACCTACAAGAACT 45.0 47.6 57.3 57.9 691 bp 1.0 65.5
trnK-UUU-rps16_p3 TCGCACTTAAAAGCCGAGTA GGTGCTCAACCTACAAGAACT 45.0 47.6 57.3 57.9 688 bp 1.0 65.5
trnK-UUU-rps16_p4 CGCACTTAAAAGCCGAGTAC GGTGCTCAACCTACAAGAACT 50.0 47.6 57.2 57.9 687 bp 1.0 65.1
trnK-UUU-rps16_p5 GTCGCACTTAAAAGCCGAGT GGTGCTCAACCTACAAGAACT 50.0 47.6 58.9 57.9 689 bp 1.0 64.5