Hotspot

rpl16

LSC · 81165-82687 · 1523 bp

Back to Hilliella

Pi 0.0218

MarkerSeek score 57.20

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1523
variable_sites: 33
pi: 0.0218
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACACATCAATTCTCGAGCCC	GTATGGAGGGAATTGCACGT	50.0	50.0	58.0	58.0	2364-2374 bp	1.0	81.3
rpl16_p2	GTCTCAATTCCCGTGGGATC	GTATGGAGGGAATTGCACGT	55.0	50.0	58.0	58.0	2623-2633 bp	1.0	81.0
rpl16_p3	ACACATCAATTCTCGAGCCC	TGCACGTGTTGAATGGATCA	50.0	45.0	58.0	58.1	2012-2022 bp	1.0	81.0
rpl16_p4	ATCGGCGATTACTAGCTCCT	GTATGGAGGGAATTGCACGT	50.0	50.0	58.1	58.0	2395-2405 bp	1.0	80.8
rpl16_p5	GTCTCAATTCCCGTGGGATC	TGCACGTGTTGAATGGATCA	55.0	45.0	58.0	58.1	2271-2281 bp	1.0	80.7