Hotspot

clpP

LSC · 69814-71875 · 2062 bp

Back to Hilliella

Pi 0.0127

MarkerSeek score 51.10

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2062
variable_sites: 26
pi: 0.0127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CGAGTACATGTTCCTCGTCG	GAACAGAAAGCAAACGACCG	55.0	50.0	58.2	58.0	2888-2897 bp	1.0	80.6
clpP_p2	CCGAGTACATGTTCCTCGTC	GAACAGAAAGCAAACGACCG	55.0	50.0	57.8	58.0	2889-2898 bp	1.0	80.6
clpP_p3	TGACATTTCGGATTGGCTGT	GAACAGAAAGCAAACGACCG	45.0	50.0	57.8	58.0	2836-2845 bp	1.0	80.5
clpP_p4	AAATGGAATTTCCCCCGCTT	CCAATACGCAATGGGGGTTA	45.0	50.0	58.0	57.9	2889 bp	0.5	59.1
clpP_p5	CCCCCGCTTAATGGATAACC	CCAATACGCAATGGGGGTTA	55.0	50.0	58.1	57.9	2878 bp	0.5	58.8