Hotspot

rpl22

LSC · 79738-80187 · 450 bp

Back to Hilaria

Pi 0.0133

MarkerSeek score 63.30

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 450
variable_sites: 6
pi: 0.0133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	ACCAAGTCTGAAACCGAGTG	TGTAGGCGGGGAATAACCTT	50.0	50.0	57.8	58.4	572-573 bp	1.0	75.8
rpl22_p2	ACCAAGTCTGAAACCGAGTG	AAGTAAAGTGTAGGCGGGGA	50.0	50.0	57.8	58.4	581 bp	0.5	55.3
rpl22_p3	ACCAAGTCTGAAACCGAGTG	AGTAAAGTGTAGGCGGGGAA	50.0	50.0	57.8	58.4	580 bp	0.5	55.3
rpl22_p4	ACCAAGTCTGAAACCGAGTG	AAAGTAAAGTGTAGGCGGGG	50.0	50.0	57.8	57.5	582 bp	0.5	54.2
rpl22_p5	CCAAGTCTGAAACCGAGTGG	AGTAAAGTGTAGGCGGGGAA	55.0	50.0	58.5	58.4	579 bp	0.5	53.3