Hotspot

ccsA

SSC · 105085-106023 · 939 bp

Back to Hilaria

Pi 0.00958

MarkerSeek score 50.30

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 939
variable_sites: 9
pi: 0.00958
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TAGACACGCTGCTCTTAGGA	TTTCGCTATCCGTTGACAGG	50.0	50.0	57.9	58.0	1317-1320 bp	1.0	80.4
ccsA_p2	TAGACACGCTGCTCTTAGGA	GGATTCTGGACCACGAGAAC	50.0	55.0	57.9	58.0	1400-1403 bp	1.0	80.3
ccsA_p3	TTAGCCGCCATGGTGAAATT	TTTCGCTATCCGTTGACAGG	45.0	50.0	58.2	58.0	1339-1342 bp	1.0	80.1
ccsA_p4	TTAGCCGCCATGGTGAAATT	GGATTCTGGACCACGAGAAC	45.0	55.0	58.2	58.0	1422-1425 bp	1.0	80.1
ccsA_p5	ATGGATTCCCCCAATCTCGA	TTTCGCTATCCGTTGACAGG	50.0	50.0	58.2	58.0	1413-1417 bp	1.0	79.9