Hotspot

ccsA-ndhD

SSC · 106024-106210 · 187 bp

Back to Hilaria

Pi 0.0489

MarkerSeek score 71.10

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 187
variable_sites: 9
pi: 0.0489
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TTCCGCATTTGTAGCTTCGA	TTGACAGGGTAGAGGCTCTC	45.0	55.0	57.9	58.1	343-346 bp	1.0	80.9
ccsA-ndhD_p2	TTCCGCATTTGTAGCTTCGA	GACAGGGTAGAGGCTCTCTT	45.0	55.0	57.9	57.8	341-344 bp	1.0	80.9
ccsA-ndhD_p3	TCCGCATTTGTAGCTTCGAT	TTGACAGGGTAGAGGCTCTC	45.0	55.0	57.7	58.1	342-345 bp	1.0	79.2
ccsA-ndhD_p4	TCCGCATTTGTAGCTTCGAT	GACAGGGTAGAGGCTCTCTT	45.0	55.0	57.7	57.8	340-343 bp	1.0	79.1
ccsA-ndhD_p5	TTCCGCATTTGTAGCTTCGA	GTTGACAGGGTAGAGGCTCT	45.0	55.0	57.9	58.4	344-347 bp	1.0	78.6