Hotspot

rpl32-trnL-UAG

SSC · 104420-104927 · 508 bp

Back to Hilaria

Pi 0.0236

MarkerSeek score 66.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 508
variable_sites: 12
pi: 0.0236
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CAACGAGCATCCAAAACCAA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.2	57.9	584-601 bp	1.0	74.3
rpl32-trnL-UAG_p2	CAACGAGCATCCAAAACCAA	AGCAGCGTGTCTACCAATTT	45.0	45.0	57.2	57.8	577-594 bp	1.0	73.8
rpl32-trnL-UAG_p3	CAACGAGCATCCAAAACCAA	GCTTGAGCACTGCTTCCTAA	45.0	50.0	57.2	58.2	598-615 bp	1.0	73.8
rpl32-trnL-UAG_p4	CAACGAGCATCCAAAACCAA	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	57.2	57.8	583-600 bp	1.0	73.7
rpl32-trnL-UAG_p5	CAACGAGCATCCAAAACCAA	CTAAGAGCAGCGTGTCTACC	45.0	55.0	57.2	57.8	582-599 bp	1.0	73.7