Hotspot

rbcL-psaI

LSC · 56301-57055 · 755 bp

Back to Hilaria

Pi 0.0212

MarkerSeek score 56.80

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 755
variable_sites: 16
pi: 0.0212
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	GACTGGAAACCGGTGGATAC	CCAAGATAACTGGAAGCCCC	55.0	55.0	58.0	57.9	1385-1422 bp	1.0	80.5
rbcL-psaI_p2	GGCTTCAGGGGGTATTCATG	CCAAGATAACTGGAAGCCCC	55.0	55.0	58.0	57.9	1656-1693 bp	1.0	80.4
rbcL-psaI_p3	GAGCTGTATTTGCGAGGGAA	CCAAGATAACTGGAAGCCCC	50.0	55.0	58.0	57.9	2029-2066 bp	1.0	80.2
rbcL-psaI_p4	GTTATACCGGTGGCTTCAGG	CCAAGATAACTGGAAGCCCC	55.0	55.0	58.1	57.9	1667-1704 bp	1.0	80.1
rbcL-psaI_p5	TATTTGCGAGGGAATTGGGG	CCAAGATAACTGGAAGCCCC	50.0	55.0	57.9	57.9	2023 bp	0.5	59.0