Hotspot

atpI-atpH

LSC · 31839-32656 · 818 bp

Back to Hilaria

Pi 0.0135

MarkerSeek score 63.90

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 818
variable_sites: 11
pi: 0.0135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TAGCCGCAGCCTATATAGGT	CTCTACAGCTTGTCCTGCAG	50.0	55.0	57.5	58.0	973-980 bp	1.0	77.0
atpI-atpH_p2	ATTTTTGCAACGTTAGCCGC	CTCTACAGCTTGTCCTGCAG	45.0	55.0	58.6	58.0	986-993 bp	1.0	76.5
atpI-atpH_p3	TAGCCGCAGCCTATATAGGT	AGCCAATCCAGCAGCAATAA	50.0	45.0	57.5	57.9	910-917 bp	1.0	75.9
atpI-atpH_p4	TAGCCGCAGCCTATATAGGT	GCAGTACCTTGACCAACTCC	50.0	55.0	57.5	58.2	954-961 bp	1.0	75.5
atpI-atpH_p5	TAGCCGCAGCCTATATAGGT	AAGCAGCAGCAATTAGTGGA	50.0	45.0	57.5	57.8	887-894 bp	1.0	75.5