Hotspot

psbM-petN

LSC · 17083-17717 · 635 bp

Back to Hilaria

Pi 0.021

MarkerSeek score 72.40

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 635
variable_sites: 13
pi: 0.021
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	ACAGCCAGCCAAAACGATTA	AGTATGGGGGAGAAGTGGAC	45.0	55.0	58.1	58.1	681-843 bp	1.0	81.3
psbM-petN_p2	ACAGCCAGCCAAAACGATTA	GTATGGGGGAGAAGTGGACT	45.0	55.0	58.1	58.1	680-842 bp	1.0	81.3
psbM-petN_p3	ACAGCCAGCCAAAACGATTA	TAGTAAGTCTCGCTTGGGCT	45.0	50.0	58.1	58.2	739-901 bp	1.0	80.9
psbM-petN_p4	ACAGCCAGCCAAAACGATTA	TATGGGGGAGAAGTGGACTC	45.0	55.0	58.1	57.8	679-841 bp	1.0	80.9
psbM-petN_p5	ACAGCCAGCCAAAACGATTA	GGGGGAGAAGTGGACTCTAG	45.0	60.0	58.1	58.2	676-838 bp	1.0	80.6