Hotspot

trnD-GUC-psbM

LSC · 15853-16977 · 1125 bp

Back to Hilaria

Pi 0.0149

MarkerSeek score 63.10

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 1125
variable_sites: 16
pi: 0.0149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TCAATTGGTTAGAGCACCGC	TAATCGTTTTGGCTGGCTGT	50.0	45.0	58.6	58.1	1241-1292 bp	1.0	79.2
trnD-GUC-psbM_p2	TCAATTGGTTAGAGCACCGC	CGTTTTGGCTGGCTGTTTTT	50.0	45.0	58.6	58.3	1237-1288 bp	1.0	77.2
trnD-GUC-psbM_p3	AATTGGTTAGAGCACCGCC	TAATCGTTTTGGCTGGCTGT	52.6	45.0	58.1	58.1	1239-1290 bp	1.0	74.6
trnD-GUC-psbM_p4	AATTGGTTAGAGCACCGCC	CGTTTTGGCTGGCTGTTTTT	52.6	45.0	58.1	58.3	1235-1286 bp	1.0	72.6
trnD-GUC-psbM_p5	TCAATTGGTTAGAGCACCGC	ATCGTTTTGGCTGGCTGTTT	50.0	45.0	58.6	59.0	1239-1290 bp	1.0	72.3