Hotspot

trnY-GUA-trnD-GUC

LSC · 15458-15778 · 321 bp

Back to Hilaria

Pi 0.0312

MarkerSeek score 61.00

Primer available yes

Label trnY-GUA-trnD-GUC

Feature Metadata

label_name: trnY-GUA-trnD-GUC
feature_type: igs
region: LSC
length_bp: 321
variable_sites: 10
pi: 0.0312
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnD-GUC_p1	GGGGACGGACTGTAAATTCG	GCGGTGCTCTAACCAATTGA	55.0	50.0	58.4	58.6	413 bp	1.0	74.7
trnY-GUA-trnD-GUC_p2	GGGACGGACTGTAAATTCGT	GCGGTGCTCTAACCAATTGA	50.0	50.0	57.6	58.6	412 bp	1.0	74.5
trnY-GUA-trnD-GUC_p3	TGGGGACGGACTGTAAATTC	GCGGTGCTCTAACCAATTGA	50.0	50.0	57.2	58.6	414 bp	1.0	71.3
trnY-GUA-trnD-GUC_p4	CTGGTTCAAATCCAGCTCGG	GCGGTGCTCTAACCAATTGA	55.0	50.0	58.9	58.6	376 bp	1.0	70.4
trnY-GUA-trnD-GUC_p5	GGGGACGGACTGTAAATTCG	GGCGGTGCTCTAACCAATT	55.0	52.6	58.4	58.1	414 bp	1.0	70.1