Hotspot

trnM-CAU-trnE-UUC

LSC · 14681-15233 · 553 bp

Back to Hilaria

Pi 0.0201

MarkerSeek score 57.20

Primer available yes

Label trnM-CAU-trnE-UUC

Feature Metadata

label_name: trnM-CAU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 553
variable_sites: 11
pi: 0.0201
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-trnE-UUC_p1	TAAGTCATCGGTTCAAATCC	TGTCCTGAACCACTAGACGA	40.0	50.0	53.0	57.7	604 bp	1.0	42.3
trnM-CAU-trnE-UUC_p2	TAAGTCATCGGTTCAAATCC	AACCACTAGACGATAGGGGC	40.0	55.0	53.0	58.6	597 bp	1.0	42.3
trnM-CAU-trnE-UUC_p3	TAAGTCATCGGTTCAAATCC	ACCACTAGACGATAGGGGC	40.0	57.9	53.0	57.9	596 bp	1.0	42.3
trnM-CAU-trnE-UUC_p4	TAAGTCATCGGTTCAAATCC	GCTGCCTCCTTGAAAGAGAG	40.0	55.0	53.0	58.3	625 bp	1.0	42.2
trnM-CAU-trnE-UUC_p5	TAAGTCATCGGTTCAAATCC	TAATACTCCCTACCCCCAGG	40.0	55.0	53.0	56.9	660 bp	1.0	42.1