Hotspot

rps16-trnQ-UUG

LSC · 5579-6711 · 1133 bp

Back to Hilaria

Pi 0.0205

MarkerSeek score 66.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1133
variable_sites: 23
pi: 0.0205
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1205-1207 bp	1.0	80.7
rps16-trnQ-UUG_p2	TCATCTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	1216-1218 bp	1.0	78.8
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	CGCATAAAACGAAATGGGGC	45.0	50.0	58.1	58.4	1267-1269 bp	1.0	77.6
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	GCATAAAACGAAATGGGGCG	45.0	50.0	58.1	58.4	1266-1268 bp	1.0	77.6
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1209-1211 bp	1.0	75.9