Hotspot

ycf1

SSC · 123369-128396 · 5028 bp

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Pi 0.00467
MarkerSeek score 42.30
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5028
variable_sites
74
pi
0.00467
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TCATTTCTTCTTCTGCCTGTGA GACTATAAAGTCGAAATCAGAAA 40.9 30.4 57.6 52.4 253-295 bp 1.0 42.5
ycf1_p2 TCATTTCTTCTTCTGCCTGTGA GACTATAAAGTCGAAATCAGAAAA 40.9 29.2 57.6 53.2 253-295 bp 1.0 42.5
ycf1_p3 CATTTCTTCTTCTGCCTGTGA GACTATAAAGTCGAAATCAGAAA 42.9 30.4 56.2 52.4 252-294 bp 1.0 42.5
ycf1_p4 TCATTTCTTCTTCTGCCTGTG GACTATAAAGTCGAAATCAGAAA 42.9 30.4 56.2 52.4 253-295 bp 1.0 42.5
ycf1_p5 TCATTTCTTCTTCTGCCTGTGA GACTATAAAGTCGAAATCAGAAAAT 40.9 28.0 57.6 53.8 253-295 bp 1.0 42.5