Hotspot

ccsA

SSC · 114032-115000 · 969 bp

Back to Hieracium

Pi 0.00243

MarkerSeek score 37.30

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 6
pi: 0.00243
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GCGGCATGCCATCTTCTAAA	TGATTCACACGGTTCTCGC	50.0	52.6	59.0	58.2	1171 bp	1.0	63.3
ccsA_p2	GCGGCATGCCATCTTCTAA	TGATTCACACGGTTCTCGC	52.6	52.6	58.3	58.2	1171 bp	1.0	60.8
ccsA_p3	CGGCATGCCATCTTCTAAAAG	TGATTCACACGGTTCTCGC	47.6	52.6	57.7	58.2	1170 bp	1.0	60.4
ccsA_p4	CGGCATGCCATCTTCTAAAA	TGATTCACACGGTTCTCGC	45.0	52.6	56.5	58.2	1170 bp	1.0	59.0
ccsA_p5	GCGGCATGCCATCTTCTAAA	GATTCACACGGTTCTCGCC	50.0	57.9	59.0	58.9	1170 bp	1.0	57.4