Hotspot

rpl32-trnL

SSC · 113029-113844 · 816 bp

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Pi 0.00862

MarkerSeek score 66.90

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 816
variable_sites: 21
pi: 0.00862
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	AAAGGAAGGGATATTGGGCG	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.6	58.0	919-1024 bp	1.0	81.7
rpl32-trnL_p2	AAAGGAAGGGATATTGGGCG	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.6	58.0	920-1025 bp	1.0	81.7
rpl32-trnL_p3	CGGCGTTAAAAGCTTTGTCA	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.6	58.0	901-1006 bp	1.0	81.5
rpl32-trnL_p4	CGGCGTTAAAAGCTTTGTCA	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.6	58.0	902-1007 bp	1.0	81.5
rpl32-trnL_p5	AAAGGAAGGGATATTGGGCG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.6	57.9	929-1034 bp	1.0	80.7