Hotspot

ndhF-rpl32

SSC · 111768-112863 · 1096 bp

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Pi 0.00574

MarkerSeek score 61.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1096
variable_sites: 20
pi: 0.00574
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	CGCCCAATATCCCTTCCTTT	50.0	50.0	58.0	57.6	1242-1296 bp	1.0	80.2
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	CGCCCAATATCCCTTCCTTT	45.0	50.0	58.0	57.6	1240-1294 bp	1.0	80.1
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	CGCCCAATATCCCTTCCTTT	45.0	50.0	58.0	57.6	1241-1295 bp	1.0	80.1
ndhF-rpl32_p4	GTTGCCGTCGGAAAAAGAAG	TGACAAAGCTTTTAACGCCG	50.0	45.0	58.0	57.6	1260-1314 bp	1.0	80.0
ndhF-rpl32_p5	TGCCGTCGGAAAAAGAAGAA	TGACAAAGCTTTTAACGCCG	45.0	45.0	58.0	57.6	1258-1312 bp	1.0	79.9