Hotspot

petA-psbJ

LSC · 63294-64109 · 816 bp

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Pi 0.00357

MarkerSeek score 55.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 816
variable_sites: 10
pi: 0.00357
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTACAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.0	57.6	950-973 bp	1.0	78.1
petA-psbJ_p2	ACGTGTACAAGGCCTTTTGT	TGTGATCGGTTTAGTAGGCG	45.0	50.0	58.0	57.4	988-1011 bp	1.0	76.4
petA-psbJ_p3	ACGTGTACAAGGCCTTTTGT	GTGATCGGTTTAGTAGGCGT	45.0	50.0	58.0	57.4	987-1010 bp	1.0	76.4
petA-psbJ_p4	TCCATTACGTGTACAAGGCC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.6	57.6	956-979 bp	1.0	75.4
petA-psbJ_p5	CCATTACGTGTACAAGGCCT	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.6	57.6	955-978 bp	1.0	75.3