Hotspot

rpl32-trnL-UAG

SSC · 120736-121905 · 1170 bp

Back to Hibiscus

Pi 0.0401

MarkerSeek score 77.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1170
variable_sites: 127
pi: 0.0401
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CGGCGTTGAAAGCTTTTTCA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.2	58.0	923-1387 bp	1.0	90.6
rpl32-trnL-UAG_p2	CGGCGTTGAAAGCTTTTTCA	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.2	58.0	924-1388 bp	1.0	90.6
rpl32-trnL-UAG_p3	CGGCGTTGAAAGCTTTTTCA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.2	57.9	933-1397 bp	1.0	89.7
rpl32-trnL-UAG_p4	AAGAAAGGATATTGGGCGGC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.3	58.0	1222-1403 bp	0.555556	69.8
rpl32-trnL-UAG_p5	AAGAAAGGATATTGGGCGGC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.3	58.0	1223-1404 bp	0.555556	69.8